《植物生理学报》 2018, 54(2): 232-236

鳄嘴花的组织培养和快速繁殖

王强^1,2, 陈冬怡², 杨国³, 陈国华^2,*, 陈红锋^2,*

¹仲恺农业工程学院, 广州510230; ²中国科学院华南植物园, 中国科学院植物资源保护与可持续利用重点实验室, 广州510650; ³绍兴文理学院生命科学学院, 浙江绍兴312000

通信作者：陈国华；E-mail: 1664238741@qq.com; h.f.chen@scbg.ac.cn

摘　要：

以鳄嘴花[Clinacanthus nutans (Burm. f.) Lindau]幼嫩茎段为外植体, 研究不同植物生长调节剂对鳄嘴花茎段腋芽诱导、丛生芽分化、增殖及生根培养的影响。结果表明: 茎段在培养基MS+1.0 mg·L^-1 BAP+0.1 mg·L^-1 NAA上诱导产生腋芽, 将腋芽转入培养基MS+1.0 mg·L^-1 BAP+0.1 mg·L^-1 NAA诱导分化丛生芽, 分化率高达93.3%; 在培养基中分别加入30 g·L^-1椰汁、30 g·L^-1香蕉、0.5 g·L^-1蛋白胨, 都有壮苗的效果; 最佳的生根培养基为MS+0.5 mg·L^-1 NAA+2.0 mg·L^-1 IBA, 生根率达90%, 且根系发达, 芽苗生长健壮。移栽至混合基质(泥炭:椰糠:珍珠岩:河沙=3:2:2:1)中, 鳄嘴花的成活率达97%。

关键词：鳄嘴花; 药用植物; 离体培养; 植株再生

收稿：2018-01-09   修定：2018-02-01

资助：中国科学院战略性先导科技专项(XDA13020603)和广东省2013年中央农业技术推广与服务补助资金项目。

Tissue culture and rapid propagation in vitro of Clinacanthus nutans

WANG Qiang^1,2, CHEN Dong-Yi², YANG Guo³, CHEN Guo-Hua^2,*, CHEN Hong-Feng^2,*

¹Zhongkai University of Agriculture and Engineering, Guangzhou 510230, China; ²Key Laboratory of Plant Resources Conservation and Sustainable Utilization, Chinese Academy of Sciences, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China; ³Shaoxing University, Academy of Life Sciences, Shaoxing, Zhejiang 312000, China

Corresponding author: CHEN Guo-Hua; E-mail: 1664238741@qq.com; h.f.chen@scbg.ac.cn

Abstract:

The regeneration system of tissue culture of Clinacanthus nutans was established by utilizing its in vitro-derived stem as explant. We analyzed the differentiations characteristics of explant, and discussed affecting factors of tissue culture of hormone and additional nutrient. The results showed the optimal proliferation medium for C. nutans was MS with 1.0 mg L^-1 BAP and 0.1 mg·L^-1 NAA. The plantlets grew well when the organics was adding, 0.5 g·L^-1 peptone, 30 g·L^-1 coconut juice and 30 g·L^-1 banana were good for cultivating strong seedling. A rooting medium composed of MS with 0.5 mg·L^-1 NAA and 2.0 mg·L^-1 IBA provided 90% rooting and vigourous plantlets. Plantlets were transplanted to a potting mixture (3:2:2:1, peat: coconut coir: perlite: sand) in trays with about 97% survival percentage.

Key words: Clinacanthus nutans; medicinal plant; tissue culture; plant regeneration